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Chromatography and Mass Spectrometry Facility

Chromatography is used to separate mixtures into individual components. In some instances, the individual components may then be identified if their elution time matches that of a standard. The amount of each component in a mixture can also be determined by comparing the area of a peak with a standard of known concentration. Gas chromatography (GC) is used with samples that are stable at elevated temperatures. Samples must be able to travel through the GC column in the gas phase. GC coupled to a mass spectrometer (GC/MS) can be used to identify unknown components in a mixture. High pressure liquid chromatography (HPLC) is used to separate compounds that are not stable when heated. Many pharmaceuticals or biomolecules fall into this category.

Available Instrumentation

  • Gas Hewlett Packard 5890 Series II gas chromatograph with FID and TC detector data station and 7673 auto-sampler (GC)
  • Hewlett Packard 5972 mass selective detector with a 5890 Series II gas chromatograph, data station and 7673 auto sampler (GC/MS)
  • Hewlett Packard 1050 high pressure liquid chromatograph (HPLC) with data station and auto sampler. Detectors include a variable wavelength UV/VIS, HP 1046A programmable fluorescence, and a 1049A electrochemical detector