G. Ekin Atilla-Gokcumen
J. Solo Assistant Professor of Chemistry
Office: 877 Natural Sciences Complex
Phone: (716) 645-4130
Fax: (716) 645-6963
B.S., Koc University, Istanbul, Turkey (2003)
Ph.D., University of Pennsylvania, Philadelphia, PA (2008)
Postdoctoral Fellow, Dana Farber Cancer Institute / Harvard Medical School, Boston, MA (2009-2013)
Studying involvement of lipids in different biological processes using liquid chromatography/mass spectrometry (LC/MS).
Identification of lipids that cells regulate during different processes
Studying the fate of these lipids in biological environment
Investigating the biological implications of changes in lipid content and localization by using fluorescent imaging and other cellular and molecular biology techniques
Lipids are a broad class of biomolecules whose primary role is to form the permeability barriers, which define cellular borders and compartments within them. Increasingly, they are recognized to play critical roles as signaling molecules both within cells and between cells, as lipids themselves, or following transformation by hydrolysis, oxidation or other modifications. As a chemical biologist, my research focuses on investigating the role of lipids and lipid-derived metabolites in different cellular processes, which I think provide a major and unexplored area of biochemistry that is ripe for discovery and therapeutic applications.
Apoptosis (programmed cell death) and senescence (permanent cessation of division) are two natural processes that terminate the proliferative life of an animal cell. They play central roles in normal aging, cancer formation and progression, and response to cancer chemotherapy. Apoptosis has been heavily studied, and protein-based pathways that regulate it are well understood. Senescence, in contrast, has only recently been recognized as a major event in the life of tissue cells, and is much less well understood. Research on apoptosis and senescence have mostly been confined to key protein players. The role of lipids in these cellular events are largely unknown, and interest has focused on a small number of molecules in the sphingolipid family. We aim to construct comprehensive maps of the lipidome at different stages of cell life cycle, identify novel lipids and infer/define their functional relevance in apoptosis and senescence. The findings of these projects will not only provide a better understanding of the nature and involvement of a complex class of compounds in fundamental biological events but could also potentially provide future therapeutic applications for aging, cancer and inflammatory disease.
Selected Recent Publications:
- Lizardo D.Y., Lin Y. T., Gokcumen O.*, Atilla-Gokcumen G.E.* (2016) Regulation of Lipids is Central to Replicative Senescence. (2017) Mol BioSystems (DOI: 10.1039/C6MB00842A)
- Matich E., Butryn D., Ghafari H., del Solar V., Camgoz E., Pfeifer B., Aga D., Haznedaroglu B.Z., Atilla-Gokcumen G.E.* (2016) Mass Spectrometry-based Metabolomics of Value-added Biochemicals from Non-model Microalgae, Algal Research 19:146-54 (*Corresponding author)
- Li N., Lizardo D., Atilla-Gokcumen G.E.* (2016) Specific Triacylglycerols Accumulate via Increased Lipogenesis During 5-FU-induced Apoptosis, ACS Chem. Biol. 11(9):2583-7 (*Corresponding author)
- Luo D., Li N., Carter K., Lin C., Geng J., Shao S., Huang W., Atilla-Gokcumen G.E., Lovell J.F. (2016) Small Amounts of Unsaturated and Porphyrin Phospholipids Can Accelerate Light-triggered Liposomal Drug Release. Small 12(22):3039-47
- Mondal N., Stolfa G., Antonopoulos A., Zhu Y., Buffone A. Jr, Atilla-Gokcumen G. E., Haslam S.M., Dell A., Neelamegham S. (2016) Glycosphingolipids on human myeloid cells stabilize E-selectin dependent rolling in the multistep leukocyte adhesion cascade Arterioscler. Thromb. Vasc. Biol. 36(4):718-27
- del Solar V., Lizardo D.Y., Li N., Hurst J., Brais C.**, Atilla-Gokcumen G.E.* (2015) Differential regulation of specific sphingolipids in colon cancer cells during staurosporine-induced apoptosis. Chem. Biol. 22(12):1662-1670 (*Corresponding author. **undergraduate researcher)
- Horner I.J., Kraut N.D., Hurst J.J., Rook A.M., Collado C.M., Atilla-Gokcumen G.E., Maziarz E.P., Liu X.M., Merchea M.M., Bright F.V. Effects of Polyhexamethylene Biguanide and Polyquaternium-1 on Phospholipid Bilayer Structure and Dynamics. J. Phys. Chem. B. 2015 Aug 20;119(33):10531-42
- Singh R.R., Lester Y., Linden K.G., Love N.G., Atilla-Gokcumen G.E., Aga D.S. (2015) Application of metabolite profiling tools and time-of flight mass spectrometry in the identification of transformation products of iopromide and iopamidol during advanced oxidation. Environ. Sci. Technol. 49(5):2983-90
- Atilla-Gokcumen, G. E, Eggert, U. S. (2015) A comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems. In D. Owen (Ed.) Methods in Membrane Lipids 1232:103-113 New York, NY:Springer
- Atilla-Gokcumen G. E.*, Muro E.*, Relat-Goberna J., Sasse S., Bedigian A.V., Coughlin, M., Garcia-Manyes, S., Eggert, U. S. (2014) Dividing cells regulate their lipid composition and localization. Cell 156, 428-439. (*Contributed equally). Recommended by Faculty 1000
- Muro E.*, Atilla-Gokcumen G.E.*, Eggert, U. S. (2014) Lipids in cell biology – how can we understand them better? Mol. Bio. Cell. 5;25(12):1819-1823 (*Contributed equally)
- Atilla-Gokcumen, G. E., Bedigian, A. V., Sasse, S., and Eggert, U. S. (2011) Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure, J Am. Chem. Soc. 133, 10010-10013.