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Thomas Szyperski

Szyperski, ThomasUB Distinguished Professor
Director, UB High-Field NMR Facility
Adjunct Senior Researcher at the Hauptman-Woodward Medical Research Institute
Office: 816 Natural Sciences Complex
Phone: (716) 645-4309
Fax: (716) 645-6963
Email: szypersk_at_buffalo.edu

GFT Projection NMR webpage: http://www.nsm.buffalo.edu/Research/GFT/

NMR 2.0 webpage: http://www.nmr2.buffalo.edu/

Education:

Prediploma Biochemistry, U Tübingen (D) 1984
Prediploma Chemistry, TU Munich (D) 1985
Diploma Chemistry, TU Munich (D) 1988
Dr. sc. nat ETH, ETH Zürich (CH) 1992
Habilitation, ETH Zürich (CH) 1998
Visiting scientist, U Auckland (NZ) 1988
Fellow of the Studienstiftung des Deutschen Volkes (1982-1988)
Fellow im Verband der Chemischen Industrie (1989-1991)

Awards:

Medaille der ETH 1992
Research Innovation Award 1999, Research Corporation
Buck-Whitney Medal 2003 of the ACS
Scientific American 50 Award 2003 for “Chemistry and Materials”
Laukien Prize 2006
2007 SUNY Chancellor’s Award for Excellence in Scholarship and Creative Activities

Specializations:

Structure determination of biological macromolecules using nuclear magnetic resonance (NMR) spectroscopy, Development of Bio-NMR techniques, Structural genomics, Metabonomics.

 

Research Summary:

Our research focuses on:

  1. Development of Bio-NMR methodology.
  2. Structure determinations of biological macromolecules using nuclear magnetic resonance (NMR) spectroscopy.
  3. Development of NMR-based structural biology in supercooled water.
  4. Investigation of cellular metabolism in support of biotechnology research.
  5. Metabonomics

NMR Instrumentation:

Varian 600 and 750 MHz spectrometers (cryogenic probes)
Access to: 500 (nanoprobe), 500 (1H/ 19F/ 13C/ 15N/ 2H-probe), 400, 300 MHz spectrometers

Selected Recent Publications:

  1. Sathyamoorthy, B., Singarapu, K.K., Garcia, A.E., Szyperski, T. (2013) Protein Conformational Space Populated in Solution Probed with Aromatic Residual Dipolar (13)C-(1)H Couplings. ChemBioChem 14:684-688. PMID:23494854
  2. Pulavarti, S.V., He, Y., Feldmann, E.A., Eletsky, A., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A., Szyperski, T. (2013) Solution NMR Structures Provide First Structural Coverage of the Large Protein Domain Family PF08369 and Complementary Structural Coverage of Dark Protochlorophyllide Oxidoreductase Complexes. J.  Struct. Funct. Genomics 14:119-126. PMID:23963952
  3. Stark, J.L., Copeland, J.C., Eletsky, A., Somerville, G.A., Szyperski, T., Powers, R. (2014) Identification of Low-molecular-weight Compounds Inhibiting Growth of Corynebacteria: Potential Lead Compounds for Anitibiotics. ChemMedChem 9:282-285. PMID:24403054
  4. Sathyamoorthy, B., Parish, D.M., Montelione, G.T., Xiao, R., Szyperski, T. (2014) Spatially Selective Heteronuclear Multiple-quantum (HMQC) Spectroscopy for Bio-molecular Studies. ChemPhysChem 15:1872-1879. PMID:24789578.
  5. Liu, G., Poppe, L., Aoki, K., Yamane, H., Lewis, J., Szyperski, T. (2014) High-quality NMR Structure of Human Anti-apoptotic Protein Domain Mcl-1(171-327) for Cancer Drug Design.
    PLoS One 9:e96521. PMID:24789074
  6. Xu, X., Pulavarti, S.V., Eletsky, A., Huang, Y.J., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T. (2014) Solution NMR Structures of Homeodomains from Human Proteins ALX4, ZHX1, and CASP8AP2 Contribute the Structural Coverage of the Human Cancer Protein Interaction Network. J. Struct. Funct. Genomics 15: 201-207. PMID:24941917
  7. Pulavarti, S.V., Eletsky, A., Huang, Y.J., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T. (2015) Polypeptide Backbone, Cb and Methyl Group Resonance Assignments of the 24 kDa Plectin Repeat Domain 6 from Human Protein Plectin. J. Biomol. NMR Assign. 9:135-138. PMID:24722902
  8. Murphy, G.S., Sathyaoorthy, B., Der, B.S., Machius, M.C., Pulavarti, S.V., Szyperski, T., Kuhlman, B. (2015) Computational de novo Design of a Four-helix Bundle Protein-DND_4HB. Protein Sci. 24:434-445. PMID:25287625
  9. Wu, X., Liu, R., Sathyamoorthy, B., Yamato, K., Liang, G., Shen, L., Ma, S., Sukumaran, D.K., Szyperski, T., Fang, W., He, L., Chen, X., Gong, B. (2015) Discrete Stacking of Aromatic Oligoamide Macrocycles. J. Am. Chem. Soc. 137:5879-5882. PMID:25909625
  10. Eletsky, A., Pulavarti, S.V., Beaumont, V., Gollnick, P., Szyperski, T. (2015) Solution NMR Experiment for Measurement of 15N-1H Residual Dipolar Couplings in Large Proteins and Supramolecular Complexes. J. Am. Chem. Soc. 137: [Epub ahead of print]. PMID:26293598

Complete List of Publications